Thermo Fisher Scientific RNF149 Monoclonal Antibody (OTI10B3), TrueMAB
Human RNF149 단백질(aa 250-383)을 항원으로 제작된 Mouse IgG1 단클론 항체. Western blot에 최적화된 TrueMAB 형식. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10B3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 250–383 of human RNF149 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step.
Suggested product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
RNF149 is an E3 ubiquitin-protein ligase that ubiquitinates BRAF, leading to its proteasomal degradation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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