
Thermo Fisher Scientific PRKY Monoclonal Antibody (OTI3E10), TrueMAB
PRKY 단백질을 인식하는 Mouse 모노클로날 항체로 Western blot, IHC, ICC에 사용 가능. Human 시료에 반응하며, Lyophilized 형태로 제공. PBS 버퍼에 8% 트레할로스 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E10 |
| Immunogen | Full-length protein expressed in 293T cell transfected with human PRKY expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
PRKY (protein kinase, Y-linked) is a member of the Ser/Thr protein kinase family, belonging to the subfamily of cAMP-dependent kinases.
Encoded by a gene located near the pseudoautosomal region on chromosome Y, PRKY contains a protein kinase domain that catalyzes ATP-dependent phosphorylation of target proteins.
PRKY and its X-linked counterpart PRKX are involved in macrophage differentiation and renal epithelial cell migration.
Mutations in PRKY or PRKX genes are associated with sex reversal disorders (XX males and XY females).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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