Thermo Fisher Scientific CDC20 Polyclonal Antibody
Rabbit polyclonal antibody targeting human CDC20 protein for research use. Validated in WB, IHC, IHC(P), and IP applications. Recommended dilution ranges and storage conditions provided. Supports studies on APC/C complex function and cell cycle regulat...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 449 and 499 of human cell division cycle 20 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Pig
Target Information
CDC20 is required for full ubiquitin ligase activity of the anaphase-promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex.
It is regulated by MAD2L1: in metaphase, the MAD2L1-CDC20-APC/C ternary complex is inactive; in anaphase, the CDC20-APC/C binary complex is active in degrading substrates.
The CDC20-APC/C complex positively regulates synaptic vesicle clustering at the presynaptic membrane in postmitotic neurons.
CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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