Thermo Fisher Scientific AKT Pan Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

Immunohistochemistry (IHC)

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 111 and 398 of Human AKT1+2+3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKT Pan,uniProtId:P31749-1,ncbiNodeId:9606,antigenRange:111-398,antigenLength:480,antigenImageFileName:PA5-31915_AKT_Pan_P31749-1_Rabbit.svg,antigenImageFileNamePDP:PA5-31915_AKT_Pan_P31749-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AKT Pan,uniProtId:P31751-1,ncbiNodeId:9606,antigenRange:111-398,antigenLength:481,antigenImageFileName:PA5-31915_AKT_Pan_P31751-1_Rabbit.svg,antigenImageFileNamePDP:PA5-31915_AKT_Pan_P31751-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:AKT Pan,uniProtId:Q9Y243-1,ncbiNodeId:9606,antigenRange:111-398,antigenLength:479,antigenImageFileName:PA5-31915_AKT_Pan_Q9Y243-1_Rabbit.svg,antigenImageFileNamePDP:PA5-31915_AKT_Pan_Q9Y243-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549388

Product Specific Information

Recommended positive controls: HA-human Akt -transfected 293T cells.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (99%), Cat (98%), Pig (99%), Chicken (100%), Sheep (99%), Rhesus Monkey (100%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.