Thermo Fisher Scientific SMYD3 Polyclonal Antibody
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SMYD3 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, Human과 Mouse에 반응. 1.23 mg/mL 농도의 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
| ChIP assay (ChIP) | Assay-dependent | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 22–411 of Human SMYD3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.23 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549392 |
Product Specific Information
- Recommended Positive Controls: DDDDK-tagged SMYD3-transfected 293T, 293T, A431, HeLa, HepG2, A549, SKOV3, HCT116
- Predicted Reactivity: Human (99%), Mouse (93%), Rat (94%), Pig (95%), Rhesus Monkey (99%), Bovine (83%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SMYD3 (histone-lysine N-methyltransferase) methylates Lys-4 of histone H3 and Lys-5 of histone H4, inducing di- and tri-methylation.
SMYD3 plays a key role in transcriptional activation as a component of the RNA polymerase complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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