Thermo Fisher Scientific MARCH4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Product Specifications

Species Reactivity

Horse, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A portion of amino acids 300-350 of human RNF174 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MARCH4,uniProtId:Q9P2E8-1,ncbiNodeId:9606,antigenRange:300-350,antigenLength:410,antigenImageFileName:PA5-23146_MARCH4_Q9P2E8-1_Rabbit.svg,antigenImageFileNamePDP:PA5-23146_MARCH4_Q9P2E8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 0.2% gelatin

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2540673

Target Information

RNF174 (MARCH4) is a multi-pass membrane protein mainly found in the membrane of Golgi apparatus. It is a complex enzyme with a RING-CH-type zinc finger. It acts as a ubiquitin-protein ligase mainly involved in third step of ubiquitin conjugation. The RINGv-type zinc finger domain is required for E3 ligase activity in which the E3 ubiquitin-protein ligase mediates ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. It is mainly expressed in brain and placenta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.