Takara MicroRNA expression vectors

These vectors provide strong miRNA expression and allow you to verify and track that expression with a fluorescent protein marker. The pmR-mCherry and pmR-ZsGreen1 Vectors couple your miRNA expression cassette to a bright red or green Living Colors fluorescent reporter, for miRNA expression you can see and select.

These vectors provide strong miRNA expression and allow you to verify and track that expression with a fluorescent protein marker. The pmR-mCherry and pmR-ZsGreen1 Vectors couple your miRNA expression cassette to a bright red or green Living Colors fluorescent reporter, for miRNA expression you can see and select.

How pmR miRNA expression vectors work

Clone your miRNA sequence into the vector’s multiple cloning site (MCS) located in the 3` untranslated region (UTR) of the fluorescent protein’s mRNA transcript. This enables both molecules to be expressed simultaneously from the vector’s strong CMV promoter.

Each vector is equipped with a powerful CMV promoter, a selectable marker, and a fluorescent protein expression cassette containing either mCherry or ZsGreen1—two of our brightest Living Colors fluorescent proteins. With these vectors, you can clone and express your favorite miRNA, and then select, sort, and/or visualize the cells in which it is expressed.

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Overview

• Strong coexpression of miRNA and a bright fluorescent protein
• Clone your miRNA into the 3` UTR of mCherry or ZsGreen1
• Simultaneously verify miRNA expression and transfection efficiency via fluorescent microscopy

More Information

Applications

• Over-expression of microRNAs to study gene or microRNA function

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.