ELK Biotechnology V-ATPase D1 rabbit pAb
V-ATPase D1 rabbit polyclonal antibody for WB and ELISA. Recognizes human, mouse, and rat V-ATPase D1 subunit. Useful for studying vacuolar ATPase-mediated organelle acidification and protein sorting. Supplied at 1 mg/ml, stored at -20°C.
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ELK Biotechnology V-ATPase D1 rabbit pAb
제품 개요
V-ATPase D1 rabbit polyclonal antibody는 진핵세포 내 소기관의 산성화를 매개하는 vacuolar ATPase 복합체의 D1 서브유닛을 인식합니다. 이 항체는 Western blot 및 ELISA 분석에 적합하며, 인간, 생쥐, 및 랫드 시료에서 반응합니다.
제품 정보
| 항목 | 내용 |
|---|---|
| Product Name | V-ATPase D1 rabbit pAb |
| Alternative Names | ATP6V0D1; ATP6D; VPATPD; V-type proton ATPase subunit d 1; V-ATPase subunit d 1; 32 kDa accessory protein; V-ATPase 40 kDa accessory protein; V-ATPase AC39 subunit; p39; Vacuolar proton pump subunit d 1 |
| Applications | WB; ELISA |
| Recommended Dilutions | WB: 1/500 - 1/2000; ELISA: 1/20000; Other applications not yet tested |
| Immunogen | Synthesized peptide derived from human V-ATPase D1 (AA range: 221–270) |
| Host Species | Rabbit |
| Storage | -20°C / 1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band | 40 kDa |
| Gene ID (Human) | 9114 |
| Human Swiss-Prot No | P61421 |
| Species Reactivity | Human; Mouse; Rat |
| Cellular Localization | Membrane; Peripheral membrane protein; Cytoplasmic side; Lysosome membrane; Cytoplasmic vesicle, clathrin-coated vesicle membrane; Localizes to centrosome and base of the cilium |
Background
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase-dependent organelle acidification is essential for protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
V-ATPase comprises a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain contains the ATP catalytic site and consists of subunits A, B, G, C, D, E, F, and H, while the V0 domain includes subunits a, c, c’, c’’, and d. The D subunit encoded by this gene is ubiquitously expressed.
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