Thermo Fisher Scientific Granulocyte-Colony Stimulating Factor (G-CSF) Recombinant Mouse Monoclonal Antibody (rCSF3, 900)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL

Product Specifications

Product Specific Information

• For IHC (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.

Target Information

G-CSF (Granulocyte colony stimulating factor) is a cytokine that stimulates production and antibacterial function of neutrophils and monocytes.
Human G-CSF is an 18.8 kDa protein with 175 amino acids. It is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts, and bone marrow cells.
G-CSF regulates proliferation, differentiation, and activation of hematopoietic cells and is expressed by various transformed cells such as carcinoma and myeloblastic leukemia cells.
It is encoded by two DNA sequences, producing a full-size high-activity and a shorter low-activity isoform.
G-CSF functions through interaction with its receptor on hematopoietic progenitors, neutrophilic granulocytes, and certain carcinoma cell lines.
Clinically, G-CSF is used in treatments for neonatal infections, acute myocardial infarction, neutropenia, severe infections, sepsis, chronic autoimmune neutropenia, acute myeloid leukemia, Sweet’s syndrome, and AIDS.
It also serves as a marker protein for various carcinomas such as bladder cancer, and dysfunction is linked to Kostmann Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.