Thermo Fisher Scientific ETV4 Polyclonal Antibody
ETV4 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P)에서 검증됨. Human과 Rat 시료에 반응하며, 합성 펩타이드를 면역원으로 사용. 친화 크로마토그래피로 정제된 1 mg/mL 액상 제품으로, -20°C에서 보관.
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- PA599226
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ETV4 (Accession P43268), corresponding to amino acid residues P91–P141. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818159 |
Product Specific Information
Antibody detects endogenous levels of total ETV4.
Target Information
ETV4 (E1A enhancer binding protein, E1AF), also known as PEA3, is a member of the Ets gene family encoding sequence-specific DNA binding proteins. This family includes mouse PU.1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2, and rat GABP-α. These proteins recognize DNA motifs containing a central 5'-GGAA-3' element.
For example, PEA3 binds the motif 5'-AGGAAG-3' (PEA-3 motif) but not 5'-AGGAAC-3', which is recognized by PU.1.
ETV4 is expressed in epithelial and fibroblastic cells but not in hematopoietic cells, distinguishing it from other Ets family members such as Ets-1, Ets-2, and Fli-1, which are primarily expressed in hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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