Thermo Fisher Scientific DCLK3 Polyclonal Antibody
Rabbit polyclonal antibody targeting human DCLK3 protein. Validated for WB, IHC(P), and ICC/IF applications. High specificity for endogenous DCLK3. Supplied as liquid form, 1 mg/mL concentration, affinity purified. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human DCLK3 (Accession Q9C098), amino acid residues T312–I362 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818160 |
Product Specific Information
Antibody detects endogenous levels of total DCLK3.
Target Information
DCLK3 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK3 mRNA, like that of DCLK1 and DCLK2, is highly expressed in adult brain, but only DCLK3 transcripts are present in liver and kidney, suggesting that DCLK3 may play additional roles beyond cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein mutated in X-linked human lissencephaly. In mouse models lacking DCX, DCLK1 expression increases slightly and compensates for DCX loss; mice deficient in both DCX and DCLK1 exhibit severe phenotypes including perinatal lethality, disrupted neocortical layering, and profound hippocampal disorganization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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