Thermo Fisher Scientific Yersinia pestis F1 Antigen Monoclonal Antibody (F81D)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10 - 1:50

View 1 publication 1 publication

Immunohistochemistry (IHC)

1:10 - 1:50

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ELISA (ELISA)

1:20 - 1:200

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Product Specifications

Published species

Bacteria

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

F81D

Immunogen

15 kDa F1 antigen.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_1018632

Product Specific Information

The MA1-7427 antibody reacts with Yersinia pestis in bacterial and infected tissue samples.

MA1-7427 has been successfully used in ELISA, Western blot, immunohistochemistry, and Immunofluorescence applications.

The MA1-7427 antibody was raised against 15 kDa F1 antigen.

Target Information

Yersinia pestis is a gram-negative coccobacillus belonging to the Enterobacteriaceae. Yersinia pestis is primarily a rodent pathogen, with humans being an accidental host when bitten by an infected rat flea. The flea draws viable Y. pestis organisms into its intestinal tract. Some Y. pestis in the flea are then regurgitated when the flea gets its blood meal thus transferring the infection to a new host. While growing in the flea, Y. pestis loses its capsular layer, which is made of the F1 (or fraction 1) capsule antigen. Most of the organisms are phagocytosed and killed by the polymorphonuclear leukocytes in the human host. A few bacilli are taken up by tissue macrophages. The macrophages are unable to kill Y. pestis and provide a protected environment for the organisms to synthesize their virulence factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.