Thermo Fisher Scientific E2F1 Polyclonal Antibody
E2F1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, ICC/IF, ELISA에 적합하며 Human, Mouse, Rat 반응성. 고순도 Affinity chromatography 정제, 안정적 PBS/glycerol 저장용액, 연구용으로 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:20–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 200–301 of human E2F1 (NP_0052161) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.31 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901584 |
Product Specific Information
- Positive Samples: HeLa, A-431, HT-29
- Immunogen sequence:
GGRLEGLTQD LRQLQESEQQ LDHLMNICTT QLRLLSEDTD SQRLAYVTCQ DLRSIADPAE QMVMVIKAPP ETQLQAVDSS ENFQISLKSK QGPIDVFLCP EE
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and in the action of tumor suppressor proteins. It is also a target of transforming proteins of small DNA tumor viruses. E2F proteins contain conserved domains including a DNA-binding domain, a dimerization domain for DP protein interaction, a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain embedded within the transactivation domain. E2F1, along with E2F2 and E2F3, has an additional cyclin-binding domain. E2F1 binds preferentially to retinoblastoma protein (pRB) in a cell-cycle dependent manner and can mediate both cell proliferation and p53-dependent or independent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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