Thermo Fisher Scientific EXT1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 467-746 of human EXT1 (NP_0001182) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EXT1,uniProtId:Q16394-1,ncbiNodeId:9606,antigenRange:467-746,antigenLength:746,antigenImageFileName:PA5-116952_EXT1_Q16394-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116952_EXT1_Q16394-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.45 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901582

Product Specific Information

Positive Samples: K-562, SW480, PC-3, Mouse liver, Mouse lung

Immunogen sequence: YYYANLGLKP PSKFTAVIHA VTPLVSQSQP VLKLLVAAAK SQYCAQIIVL WNCDKPLPAK HRWPATAVPV VVIEGESKVM SSRFLPYDNI ITDAVLSLDE DTVLSTTEVD FAFTVWQSFP ERIVGYPARS HFWDNSKERW GYTSKWTNDY SMVLTGAAIY HKYYHYLYSH YLPASLKNMV DQLANCEDIL MNFLVSAVTK LPPIKVTQKK QYKETMMGQT SRASRWADPD HFAQRQSCMN TFASWFGYMP LIHSQMRLDP VLFKDQVSIL RKKYRDIERL

Target Information

Hereditary multiple exostoses (HME) is an autosomal dominant disorder characterized by the formation of exostoses (EXT), which are cartilage-capped bony protuberances mainly located on long bones. Two proteins associated with EXT, EXT1 and EXT2, form homo/heteromeric complexes in vivo, which leads to the accumulation of both proteins in the Golgi apparatus. EXT1 and EXT2 are endoplasmic reticulum-localized type II transmembrane glycoproteins that possess, or are tightly associated with, glycosyltransferase activities involved in the polymerization of the glycosaminoglycan, heparan sulfate (HS). EXT2 is a protein that harbors the D-glucuronyl (GlcA) and N-acetyl-D-glucosaminyl (GlcNAc) transferase activities required for biosynthesis of HS. EXT1 rescues defective HS biosynthesis and elevates low GlcA and GlcNAc transferase levels in mutated cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.