Thermo Fisher Scientific SOX9 Recombinant Rabbit Monoclonal Antibody (5Y4J8)
SOX9 단백질을 인식하는 토끼 단일클론 항체로, Western blot, IHC, ICC, ELISA, IP에 사용 가능. 인간, 마우스, 랫트 반응성. HEK293 세포 발현, Affinity Chromatography 정제. 핵에서 SOX9 발현 검출에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:200–1:2,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5Y4J8 |
| Immunogen | Recombinant fusion protein containing amino acids 169–300 of human SOX9 (P48436) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.06 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911869 |
Product Specific Information
Positive test controls include: HepG2, HeLa.
Localization: Nucleus
Immunogen sequence:
HPDYKYQPRR RKSVKNGQAE AEEATEQTHI SPNAIFKALQ ADSPHSSSGM SEVHSPGEHS GQSQGPPTPP TTPKTDVQPG KADLKREGRP LPEGGRQPPI DFRDVDIGEL SSDVISNIET FDVNEFDQYL PP
Target Information
SOX9 plays a crucial role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates expression of genes acting as a transcription factor.
Translocation of this gene can cause campomelic dysplasia. It contributes to the formation of testes from fetal gonads and influences prostate epithelium development.
In prostate cancer, SOX9 supports tumor growth and invasion. It also plays a role in neural crest precursor formation and melanin production.
Expression detected in adult testis, adult heart, and fetal brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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