Thermo Fisher Scientific SQSTM1 Recombinant Rabbit Monoclonal Antibody (5X4R1)
SQSTM1/p62 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합합니다. HEK293 세포에서 발현된 재조합 항체이며, 고순도 친화 크로마토그래피 정제 및 안정한 액상 보관용 PBS 버퍼 구성으로 높은 재현성과 안정성을 제공합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:80,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5X4R1 |
| Immunogen | Synthetic peptide corresponding to amino acids 341–440 of human SQSTM1/p62 (UniProt ID: Q13501) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.10 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911867 |
Product Specific Information
- Positive test controls: 293T, HeLa, L929, C2C12, PC-12
- Subcellular localization: Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, Autophagosome
Immunogen sequence:
LSSKEVDPST GELQSLQMPE SEGPSSLDPS QEGPTGLKEA ALYPHLPPEA DPRLIESLSQ MLSMGFSDEG GWLTRLLQTK NYDIGAALDT IQYSKHPPPL
Target Information
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-κB) signaling pathway. It functions as a scaffolding/adaptor protein with TNF receptor-associated factor 6 to mediate NF-κB activation in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified. Mutations in this gene are associated with sporadic and familial Paget disease of bone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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