Thermo Fisher Scientific MIP-4 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived, 7.8 kDa Recombinant Human MIP-4 (CCL18) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PARC,uniProtId:P55774-1,ncbiNodeId:9606,antigenRange:22-89,antigenLength:89,antigenImageFileName:500-P108BT-500UG_PARC_P55774-1_Rabbit.svg,antigenImageFileNamePDP:500-P108BT-500UG_PARC_P55774-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

AA Sequence of recombinant protein: QVGTNKELCC LVYTSWQIPQ KFIVDYSETS PQCPKPGVIL LTKRGRQICA DPNKKWVQKY ISDLKLNA.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-4 (CCL18). Anti-Human MIP-4 (CCL8)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human MIP-4 (CCL18) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MIP-4 (CCL18) (500-P108) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-4 (CCL18).

Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P108BT-1MG will be provided as 2 x 500 µg

Target Information

Macrophage Inflammatory Protein-4 (MIP-4), also called CCL18, is a chemokine expressed in the lymph nodes, lungs, placenta and bone marrow. Although the MIP-4s receptor remains undetermined, MIP-4 is known to act as a chemoattractant for activated and non-activated T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.