Thermo Fisher Scientific MIP-4 Polyclonal Antibody, PeproTech
Human MIP-4(CCL18) 검출용 Rabbit Polyclonal Antibody로 ELISA, Western Blot, Immunohistochemistry 등에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| ELISA | 0.5–2.0 µg/mL | 2 publications |
| Immunostaining (IS) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human MIP-4 (CCL18) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929228 |
Product Specific Information
AA Sequence of Recombinant Protein:
QVGTNKELCC LVYTSWQIPQ KFIVDYSETS PQCPKPGVIL LTKRGRQICA DPNKKWVQKY ISDLKLNA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-4 (CCL18). Anti-Human MIP-4 (CCL18) specific antibody was purified by affinity chromatography employing an immobilized Human MIP-4 (CCL18) matrix.
Sandwich ELISA:
To detect Human MIP-4 (CCL18) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIP-4 (CCL18) (500-P108Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human MIP-4 (CCL18).
Western Blot:
To detect Human MIP-4 (CCL18) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-4 (CCL18) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Macrophage Inflammatory Protein-4 (MIP-4), also called CCL18, is a chemokine expressed in lymph nodes, lungs, placenta, and bone marrow. Although the MIP-4 receptor remains undetermined, MIP-4 acts as a chemoattractant for activated and non-activated T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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