Norgen LowRanger 100 bp DNA Ladder
11개의 명확한 DNA 밴드를 포함한 100 bp 단위의 Ladder로, 500 bp 기준 밴드가 강화되어 시각적 판독이 용이함. 빠른 전기영동과 정량 분석에 적합하며, 안정적이고 사용 준비 완료 상태로 제공됨. 실온 안정성 및 장기 보관 시 -20°C 권장.
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Norgen LowRanger 100 bp DNA Ladder
SKU: 11500
Ideal for fast running times and accurate visual determination.
Features and Benefits
- Ready-to-use
- Quantitative
- Highly stable
- Precise
- Eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
- Higher intensity reference band at 500 bp
The Norgen LowRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. It contains eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments, with a higher intensity reference band at 500 bp. This ladder is ideal for fast running times and accurate visual determination.
Contents
1 mL of premixed DNA ladder (0.5 µg/10 µL) in loading buffer (10 mM EDTA, 10% glycerol, 0.015% bromophenol blue, 0.17% SDS).
Ladder Properties
| Fragment | Size (bp) | Mass (ng) |
|---|---|---|
| 1 | 2000 | 110 |
| 2 | 1500 | 83 |
| 3 | 1000 | 55 |
| 4 | 800 | 44 |
| 5 | 700 | 39 |
| 6 | 600 | 33 |
| 7 | 500 | 55 |
| 8 | 400 | 22 |
| 9 | 300 | 17 |
| 10 | 200 | 22 |
| 11 | 100 | 22 |
Additional Data from Image OCR:
| bp | ng/10µL |
|---|---|
| 2000 | 110 |
| 1500 | 83 |
| 1000 | 55 |
| 800 | 44 |
| 700 | 39 |
| 600 | 33 |
| 500 | 55 |
| 400 | 22 |
| 300 | 17 |
| 200 | 22 |
| 100 | 22 |
10 µL on 1% agarose
10 µL on 1.2% agarose
Recommended Use
Mix thoroughly. For best results, load 10 µL of DNA ladder per well.
For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5 µg/mL ethidium bromide for 30–40 minutes.
The table above shows the size and mass for each band based on 10 µL ladder per well.
Storage
Stable at room temperature. For longer-term storage, -20°C is recommended.
This ladder was standardized using 10 µL of DNA per lane on a 0.8 cm thick, 13 × 15 cm, 1.0% agarose gel run in TAE buffer.
Documentation
Protocols
Application Notes
Safety Data Sheets
Citations
| Title | Citation | Authors |
|---|---|---|
| Somatic embryogenesis in Vaccinium vitis-idaea L.: a high-fidelity micropropagation approach producing true-to-type plants with enhanced phytochemical profiles | Plant Cell, Tissue and Organ Culture (PCTOC) 2025 | Sayani Kundu, Rajesh Barua, H. Dawn Marshall, Yaw L. Siow, Abir U. Igamberdiev & Samir C. Debnath |
| Molecular analysis of Toxoplasma gondii in samples of açaí (Euterpe oleracea) ice cream purchased from commercial establishments | Food Science and Technology 2025 | Beatriz Januário Alencar et al. |
| Structural pattern and genetic diversity in blueberry (Vaccinium) clones and cultivars using EST-PCR and microsatellite markers | Genetic Resources and Crop Evolution 2017 | Tailor, S., Bykova, N. V., Igamberdiev, A. U., & Debnath, S. C |
| Alternative Splicing in Adhesion- and Motility-Related Genes in Breast Cancer | International J of Molecular Sciences 2016 | Rosanna Aversa et al. |
| Vertebral osteomyelitis associated with single and mixed bacterial infection in broilers | Avian Pathology 2016 | Braga, J. F. V. et al. |
| Propagation Methods Affect Fruit Morphology and Antioxidant Properties but Maintain Clonal Fidelity in Lowbush Blueberry | HortScience 2015 | JC Goyali, AU Igamberdiev, SC Debnath |
| Rethinking refugia: Tree topology, divergence dates, and demographic history trace the distribution of the endangered Plymouth gentian (Sabatia kennedyana) from the Pleistocene glaciation to present day | American Journal of Botany 2015 | A Suarez-Gonzalez et al. |
| Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes | BioMed Research International 2014 | K Bohme et al. |
| Toll-like receptor 2 and 4 expression in the pregnant and non-pregnant human uterine cervix | Journal of Reproductive Immunology 2014 | BS Lashkari, S Shahana, DO Anumba |
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