Norgen CleanAll DNA/RNA Clean-Up and Concentration Micro Kit

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Norgen CleanAll DNA/RNA Clean-Up and Concentration Micro Kit

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RNA와 DNA를 빠르고 효율적으로 정제, 농축 및 클린업하는 스핀 컬럼 키트. 모든 크기의 RNA와 DNA를 정제 가능하며, 엔도톡신 제거 기능으로 트랜스펙션용 고품질 샘플 확보. 20분 내 10회 정제 완료, 높은 회수율(≥90%) 제공.

브랜드: Norgen
카테고리: Genomic DNA Purification Systems and Kits
판매단위: pk

#Norgen #RNA/DNA Reagents #R #Reagents

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Norgen · Norgen CleanAll DNA/RNA Clean-Up and Concentration Micro Kit

Norgen CleanAll DNA/RNA Clean-Up and Concentration Micro Kit

SKU: 23800
Rapid and efficient purification, cleanup and concentration of RNA or DNA.

Features and Benefits

Cleans up both RNA and DNA from enzymatic reactions, labeling, etc.
Purifies all sizes of RNA, from large mRNA down to microRNA (miRNA).
Purifies all sizes of DNA, from small PCR products to plasmids to genomic DNA.
Removes endotoxins for transfection or injection-ready RNA/DNA.
Rapid and efficient spin-column format (20 minutes).
Based on Norgen’s proprietary resin separation matrix.

Norgen’s CleanAll DNA/RNA Clean-Up and Concentration Micro Kit offers a rapid method for purification and concentration of RNA or DNA from various isolation methods or upstream applications. It can replace organic extraction or ethanol precipitation for enzymatic reaction cleanup.
The kit purifies RNA from phenol/guanidine-based protocols or enzymatic reactions such as DNase treatment, labeling, and in vitro transcription, while effectively removing endotoxins.
It also cleans up DNA from digestions, ligations, PCR, labeling, and modification reactions, with endotoxin removal down to 0.1 EU/µg DNA or less.
Purifies all sizes of RNA (mRNA, rRNA, miRNA, siRNA) and DNA (PCR products, plasmids, genomic DNA). Smaller fragments and oligonucleotides can be purified using an alternative protocol.

Supporting Data

Figure 1. High Recoveries and Efficient Endotoxin Removal
High recoveries of DNA are maintained even during endotoxin removal.

Panel A: Comparison of PCR Purification Kit and CleanAll Kit shows >90% recovery for both.
Panel B: Endotoxin levels reduced from 0.75 EU/µg to <0.05 EU/µg, achieving ~95% removal efficiency.

[패널 A 데이터 요약]

항목	값(그래프상)
제목	% Recovery
x축 레이블	PCR Purification Kit / CleanAll Kit
y축 범위	0 ~ 100
막대 수	2
회수율	약 90% / 약 95%

[패널 B 데이터 요약]

항목	값(그래프상)
제목	Average EU/µg plasmid
x축 레이블	Before / After
y축 범위	0 ~ 0.9
막대 수	2
엔도톡신 수치	약 0.8 → 약 0.05 이하

Figure 2. Clean-Up of RNA with High Recovery
RNA cleanup after DNase treatment shows high recovery and integrity.
Lane 1: RNA input; Lanes 2–5: purified RNA using CleanAll Kit.
All lanes show intact RNA bands with high yield.

[이미지 텍스트 요약]
Lane 번호: 1, 2, 3, 4, 5 (젤 전기영동 결과 표시)

Kit Specifications

항목	내용
Column Binding Capacity	50 μg RNA / 10 μg DNA
Maximum Column Loading Volume	600 μL
Minimum Elution Volume	20 μL
Time to Complete 10 Purifications	20 minutes
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Size of DNA Purified	≥100 bp*
Average Recovery	≥90% for RNA / ≥90% for DNA 100 bp–10 kbp / ≥75% for DNA ≥10 kbp

* Smaller fragments or oligonucleotides can be purified with an alternative protocol.

Storage Conditions and Product Stability

All solutions should be tightly sealed and stored at room temperature.
Stable for 2 years from shipment date.

Kit Components

Component	Cat. 23800 (50 preps)
Binding Buffer H	60 mL
Wash Solution K	27 mL
Elution Buffer L	15 mL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Documentation

FAQs (Micro Kit)

Why do I have poor DNA recovery?

Possible causes include inefficient binding, incorrect ethanol addition, incomplete wash, improper elution buffer use, or high sample viscosity.

Why is purified DNA not performing well?

Residual salts may remain if washing is insufficient. Spin for 2 minutes during the wash step.

Why does DNA have high endotoxin levels?

Use provided Elution Buffer L; repeat purification if input DNA has very high endotoxin levels.

Why do I have poor RNA recovery?

Possible causes: clogged column, alternative elution buffer, missing ethanol addition to lysate or wash solution.

Column clogging causes

Excess RNA or genomic DNA, or low centrifuge temperature (<15℃) can cause clogging.

Why is eluted RNA degraded?

RNase contamination, slow procedure, or improper storage. Store RNA at –70℃ for long-term.

Why does RNA not perform well?

Incomplete washing or ethanol carryover can interfere with downstream applications.

DNA contamination in RNA

Reduce starting material amount; perform DNase I digestion before cleanup.

EDTA concentration in Elution Buffer L

Elution Buffer L is EDTA-free.

Citations (Selected)

Title	Journal / Year	Authors
Terrestrial runoff is an important source of biological ice-nucleating particles in Arctic marine systems	Atmospheric Chemistry and Physics, 2025	Corina Wieber et al.
From the Mountain to the Valley: Drivers of Groundwater Prokaryotic Communities along an Alpine River Corridor	Microorganisms, 2023	Alice Retter et al.
Microbial Community Succession Along a Chronosequence in Constructed Salt Marsh Soils	Microbial Ecology, 2023	Carol Kim et al.
Bioengineering novel chimeric microRNA-34a for prodrug cancer therapy	J. Pharmacol. Exp. Ther., 2015	W. Wang et al.