Thermo Fisher Scientific Renal Cell Carcinoma (Carbonic Anhydrase IX) Recombinant Rabbit Monoclonal Antibody (CA9, 2993R)
Renal Cell Carcinoma 연구용 Carbonic Anhydrase IX 타깃 재조합 토끼 단클론 항체. IHC(P) 검증 완료, HEK293 발현 시스템 사용. 고순도 Protein A/G 정제, 4°C 보관 및 연구용 전용.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Horse, Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CA9, 2993R |
| Immunogen | Recombinant full-length human CA9 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Carbonic anhydrase (CA) is an enzyme that catalyzes the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues.
CA IX is highly inducible under hypoxic conditions via HIF-1α and serves as a reliable histochemical marker of hypoxia. Carbonic anhydrases regulate pH in normal tissues by controlling hydrogen ion (H⁺) flux, and inhibition of CA IX can increase cell death under hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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