Thermo Fisher Scientific Renal Cell Carcinoma (Carbonic Anhydrase IX) Monoclonal Antibody (CA9/4019)
인간 Carbonic Anhydrase IX 단백질을 인식하는 마우스 모노클로날 항체로, 신세포암 연구에 적합합니다. Western blot, IHC, Flow cytometry 등 다양한 응용에 사용 가능하며, 단백질 A/G로 정제된 액상 형태입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CA9/4019 |
| Immunogen | Recombinant human CA9 protein fragment (around aa 314–410) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Carbonic anhydrase (CA) is an enzyme that facilitates the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in mammalian tissues. Many genes are inducible by hypoxia via HIF-1 alpha, and CA IX is one of the most inducible due to its stability and membrane localization. Carbonic anhydrases regulate pH in normal tissues by controlling hydrogen ion (H⁺) flux. Since pH plays a key role in cell death under hypoxia, inhibition of CA IX increases cell death in such conditions. Therefore, CA IX serves as a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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