Thermo Fisher Scientific Fumarase Monoclonal Antibody (GT548)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

GT548

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735187

Product Specific Information

Positive Control: 293T, mouse liver, rat liver

Predicted Reactivity: Zebrafish (90%), Pig (98%), Chicken (91%), Sheep (99%), Chimpanzee (99%), Bovine (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.