Thermo Fisher Scientific Phospho-CDK2 (Tyr15) Recombinant Rabbit Monoclonal Antibody (2C4)
인간 CDK2 Tyr15 인산화 형태를 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, IHC, ICC, ELISA, IP 등 다양한 분석에 적합. 고순도 Protein G 정제, 액상 형태로 제공되며 안정적 저장 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:80 | - |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | 1:200–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2C4 |
| Immunogen | A synthesized peptide derived from human Phospho-CDK2 (Y15) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2811944 |
Target Information
CDK2/cyclinO is a member of the cyclin-dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene, which is localized exclusively in the nucleus. CyclinO shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and are turned over during the course of the cell cycle. The levels of cyclin A–CDK2 are maximal at the G1/S transition, and both CDK2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as a substrate for CDK2–cyclin E and/or CDK2–cyclin A in vivo. CDK2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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