Thermo Fisher Scientific Adeno-Associated Virus Capsid Protein Polyclonal Antibody, FITC
AAV 캡시드 단백질을 인식하는 FITC 결합 폴리클로날 항체. Western blot, ELISA, IP에 적합. 항원 친화 크로마토그래피로 정제됨. 498/517 nm 형광 특성. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids within region 200–250 on rat 5-hydroxytryptamine receptor 1A protein |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Proprietary buffer with 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at –20°C avoiding freeze/thaw cycles; store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Capsid protein self-assembles to form an icosahedral capsid with T=1 symmetry, approximately 22 nm in diameter, consisting of 60 copies of three size variants (VP1, VP2, VP3) differing in their N-terminus. The capsid encapsulates the genomic ssDNA. It binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as a coreceptor for virion attachment to target cells. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of the VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signals. The VP1 N-terminus may function as a lipolytic enzyme to breach the endosomal membrane during host cell entry and facilitate viral transport to the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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