Thermo Fisher Scientific Adeno-Associated Virus Capsid Protein Polyclonal Antibody
AAV 캡시드 단백질을 인식하는 토끼 폴리클로날 항체로, WB, ELISA, IP에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 4°C 단기 보관 및 -20°C 장기 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids within region 200–250 on rat 5-hydroxytryptamine receptor 1A protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Proprietary buffer with 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Capsid protein self-assembles to form an icosahedral capsid with T=1 symmetry, approximately 22 nm in diameter, consisting of 60 copies of three size variants of the capsid protein VP1, VP2, and VP3, which differ in their N-terminus.
The capsid encapsulates the genomic ssDNA, binds to host cell heparan sulfate, and uses host ITGA5-ITGB1 as a coreceptor for virion attachment. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis.
Binding to the host receptor also induces capsid rearrangements, leading to surface exposure of the VP1 N-terminus, including its phospholipase A2-like region and putative nuclear localization signals. VP1 N-terminus may function as a lipolytic enzyme to breach the endosomal membrane during host cell entry and may contribute to viral transport to the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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