Thermo Fisher Scientific Phospho-eIF4A2 (Tyr49) Polyclonal Antibody
Phospho-eIF4A2 (Tyr49) 항체로 인산화된 eIF4A2 단백질을 특이적으로 검출. Western blot에 적합하며, 사람·생쥐·랫트 반응성. 고순도 rabbit polyclonal IgG로 PBS/glycerol buffer에 보관. 연구용으로 단백질 합성 조절 및 신호전달 연구에 활용 가능.
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Thermo Fisher Scientific Phospho-eIF4A2 (Tyr49) Polyclonal Antibody
Applications
- Western Blot (WB): 1:500–1:2,000 dilution
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human EIF4A2 (Accession Q14240), corresponding to amino acid residues around phosphorylated Tyr49 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816590 |
Product Specific Information
This antibody detects endogenous levels of eIF4A2 only when phosphorylated at Tyr49.
Target Information
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. As a subunit of the eIF4F complex, eIF4A2 acts as a protein-synthesis initiation factor and plays a key role in mRNA binding to the 43S pre-initiation complex of ribosomes.
Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates genomic RNA synthesis of NS5B by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most normal tissues, though in variable amounts.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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