Thermo Fisher Scientific ATP5B Monoclonal Antibody (OTI3H8), TrueMAB
ATP5B 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. 인간 시료에 반응하며, 동결건조 형태로 제공됩니다. 고순도의 친화 크로마토그래피 정제 제품으로 연구용에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H8 |
| Immunogen | Full length human recombinant protein of human ATP5B produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ATP synthase is a highly conserved enzyme complex found in plants, fungi, bacteria, and animals. It catalyzes the reversible conversion of ADP and phosphate to ATP, driven by proton flux across membranes during electron transport.
The enzyme consists of two major sections:
- F1 ATP-ase (soluble): composed of α, β, γ, δ, and ε subunits
- F0 ATP-ase (membrane-embedded): composed of a, b, and c subunits
Usage Information
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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