Thermo Fisher Scientific ATP5B Monoclonal Antibody (OTI4E5), TrueMAB
ATP5B 단백질을 인식하는 고특이성 마우스 단클론 항체로, Western blot, ICC/IF, Flow Cytometry에 적합. HEK293T 세포에서 생산된 full-length 인간 ATP5B 단백질을 면역원으로 사용. 무보존제, 동결건조 형태로 안정적이며 연구용으로 최적화됨.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4E5 |
| Immunogen | Full length human recombinant protein of human ATP5B produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ATP synthase is a highly conserved transmembrane enzyme found in plants, fungi, bacteria, and animals.
It catalyzes the reversible conversion of ADP and phosphate to ATP, driven by proton flux across the membrane.
The enzyme consists of two major sections:
- F1 ATPase (soluble): composed of alpha, beta, gamma, delta, and epsilon subunits.
- F0 ATPase (membrane embedded): composed of a, b, and c subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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