Thermo Fisher Scientific RFC4 Monoclonal Antibody (OTI1A8), TrueMAB
RFC4 단백질을 표적으로 하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, ICC/IF에 사용 가능. 다양한 종에서 반응하며, 고순도 친화 크로마토그래피로 정제됨. 동결건조 형태로 제공되며, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:4000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A8 |
| Immunogen | Full length human recombinant protein of human RFC4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Human Replication Factor C (RFC) is a hetero-pentameric protein composed of the p140, p40, p38, p37, and p36 subunits.
RFC plays a key role in loading the DNA polymerase delta processivity factor (PCNA, Proliferating Cell Nuclear Antigen) onto a primed DNA template during DNA synthesis and repair.
It may also be involved in the DNA damage checkpoint response with a PCNA-like hetero-trimer consisting of Hus1, Rad1, and Rad9.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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