Thermo Fisher Scientific SEMA3F Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Product Specifications

Product Specific Information

• Recommended positive controls: A549, HeLa, H1299, HCT116
• Predicted reactivity: Mouse (95%), Rat (93%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The semaphorins are a family of proteins involved in signaling. All family members contain a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]).
Sequence comparisons have grouped secreted semaphorins into three general classes, all of which also include an immunoglobulin domain.
The semaphorin III family — including human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E — all have a basic domain at the C terminus.
Chicken collapsin contributes to axonal pathfinding during development by inhibiting growth cone extension (Luo et al., 1993 [PubMed 8402908]) through interaction with a collapsin response mediator protein (CRMP62) (Goshima et al., 1995 [PubMed 7637782]).
SEMA3F is a secreted member of the semaphorin III family.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.