Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), eBioscience
쥐 IFN-γ를 인식하는 중화용 단일클론 항체로, ELISA·Flow Cytometry·Western blot 등 다양한 면역분석에 사용 가능. 높은 순도(>90%)와 안정적 액상 형태로 제공되며, 4°C 보관. 다양한 형광 및 효소 표지 형식으로 선택 가능.
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Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 |
| Immunohistochemistry (IHC) | - | 6 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 |
| Immunocytochemistry (ICC/IF) | - | 3 |
| Flow Cytometry (Flow) | - | 286 |
| ELISA | 0.25–1 µg/mL | 19 |
| Neutralization (Neu) | - | 10 |
| Functional Assay | - | 16 |
| T-Cell Activation (TCA) | - | 1 |
| In vitro Assay (IV) | - | 3 |
| Miscellaneous PubMed (Misc) | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | XMG1.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468467 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugates
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 660
- FITC
- Functional Grade
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Custom conjugation available upon request
Product Specific Information
The XMG1.2 antibody reacts with mouse interferon (IFN) gamma and functions as a neutralizing antibody. Mouse IFN gamma is a 20 kDa cytokine produced by activated T, B, and NK cells, exhibiting antiviral and antiparasitic activity. It inhibits proliferation of normal and transformed cells and modulates immune responses through high-affinity IFN gamma receptors. Glycosylation does not affect its biological activity.
Applications Reported:
Used in ELISA, flow cytometry (intracellular staining), immunoblotting (WB), and neutralization assays.
Applications Tested:
Validated as a capture antibody in sandwich ELISA with biotin R4-6A2 (13-7312) for detection and recombinant mouse IFN gamma (39-8311) as standard. Recommended ELISA capture range: 0.25–1 µg/mL. Excess antibody may reduce sensitivity. Include a standard curve (2000 pg/mL–15 pg/mL) in each plate.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced mainly by T lymphocytes and NK cells. It is a dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits. Biological activity is species-specific; human IFN gamma does not cross-react with mouse. Functions include antiviral and antiproliferative activity, MHC induction, macrophage activation, and enhanced immunoglobulin secretion. It acts via IFN gamma receptor I/II and the JAK-STAT pathway. Human IFN gamma shares ~40% sequence homology with mouse IFN gamma. Mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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