Thermo Fisher Scientific IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eBioscience
인간 IL-21을 인식하는 단클론 항체로 NK 및 T 세포 활성화 연구에 적합. ELISA 캡처용으로 검증되었으며 1–4 µg/mL 농도 범위에서 사용 가능. 고순도(>90%)로 정제된 액상 형태이며 PBS 완충액 내 저장. 연구용으로만 사용 가능.
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Thermo Fisher Scientific IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry | - |
| ELISA | 1–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio3A3-N2 (3A3-N2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865275 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein closely related to IL-2 and IL-15, expressed in activated CD4+ cells. It stimulates proliferation of B cells via CD40 antigen crosslinking, promotes bone marrow progenitor cell proliferation, and induces NK-cell marker CD56 expression in presence of IL-15.
Applications Reported:
Used for ELISA capture in IL-21 analysis.
Applications Tested:
Validated as capture antibody in sandwich ELISA for human IL-21 detection, in combination with biotin-labeled eBio2B2-G20 (Product #13-7218-81) and recombinant human IL-21 standard (Product #39-8219-65).
Recommended ELISA capture concentration: 1–4 µg/mL.
Include standard curve (4000 pg/mL – 60 pg/mL) in each plate.
Target Information
IL-21 is a 17 kDa immunomodulatory cytokine mainly produced by NKT, Th17, and TFH cells.
In TFH cells, IL-21 triggers autocrine signaling via IL-21R and STAT3, activating transcription factor Bcl6. It is critical for TFH cell development and function, promoting B cell differentiation into plasma and memory cells, stimulating IgG production, and inducing apoptosis in naive B cells.
In Th17 cells, IL-21 regulates IL-23R expression through STAT3, IRF4, and RORγt, promoting maturation and maintenance under IL-23 stimulation. It also downregulates Foxp3.
IL-21-deficient mice show protection against chemically induced colitis, while elevated IL-21 levels are observed in colitis models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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