Thermo Fisher Scientific HIV1 Tat Monoclonal Antibody (7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

ELISA (ELISA)

0.2-1 µg/mL

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

ChIP assay (ChIP)

1-3 µL

-

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7

Immunogen

Recombinant Tat protein of HIV-1 BH10 purified from E. coli

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_962124

Product Specific Information

This antibody reacts with HIV-1 Tat BH10, IIIB strains. This antibody reacts with the rTat consensus sequences of HIV-1 subtypes A (PNLEPWH), B (PRLEPWK) and C (PNLEPWN). It has been mapped to amino acids 6-12 (PRLEPWK - B subtype a.a. consensus).

MA1-71509 can be used for chromatin immunoprecipitation (ChIP) analysis of HIV1 Tat.

This clone may also be referred to as 7 (02-002).

Target Information

HIV-1 Tat (Trans-Activator of Transcription) consists of 86 to 101 amino acids depending on the subtype. Tat is critical to HIV reproduction by way of compensating for a defect in its genome: the HIV RNA initially has a hairpin-structured portion which prevents full transcription from occurring. However, a small number of RNA transcripts will be made, which allow the Tat protein to be produced. Tat then binds to and phosphorylates cellular factors, eliminating the effect of the hairpin RNA structure and allowing transcription of the HIV RNA. This increases the rate of transcription, providing a positive feedback cycle. The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.