Thermo Fisher Scientific BACE2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:200

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C P(503) E V V N D E S S L V R H R W K(518) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BACE2,uniProtId:Q9Y5Z0-1,ncbiNodeId:9606,antigenRange:503-518,antigenLength:518,antigenImageFileName:PA1-754_BACE2_Q9Y5Z0-1_Rabbit.svg,antigenImageFileNamePDP:PA1-754_BACE2_Q9Y5Z0-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_326006

Product Specific Information

PA1-754 detects human and mouse beta-secretase 2 (BACE2) from transfected cells. This product has recently been remanufactured in new rabbits as Cat. # PA1-1754.

PA1-754 has been successfully used in Western blot and ICC/IF procedures. By Western blot this antibody detects an ~58 kDa protein representing BACE2 from SY-SY5Y cells overexpressing the human gene.

The PA1-754 immunogen is amino acid residues 503-518 from human BACE2. This sequence is completely conserved in mouse. The PA1-754 immunizing peptide (Cat. # PEP-167) is available for use in neutralization and control experiments.

Target Information

BACE2 or Beta Secretase 2 is responsible for the proteolytic processing of the amyloid precursor protein (APP). BACE2 cleaves APP between residues 690 and 691. This cleavage leads to the generation and extracellular release of beta-cleaved soluble APP. BACE2 is also know to cleave APP between residues 671 and 672. Accumulation of APP is a signature of Alzheimer disease and frequent complication of adult Down syndrome patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.