Thermo Fisher Scientific Desmin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:75-1:150

-

Immunohistochemistry (Frozen) (IHC (F))

1:75-1:150

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human Desmin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3 mg/mL

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_780120

Product Specific Information

PA1-27003 detects Desmin from all mammal samples.

PA1-27003 has been successfully used in immunofluorescence, immunohistochemistry (frozen), immunohistochemistry (paraffin), and Western blot procedures. Antigen retrieval is required for immunostaining.

The PA1-27003 immunogen is full length human protein.

Target Information

Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.