Thermo Fisher Scientific Insulin Polyclonal Antibody
Thermo Fisher Scientific의 Insulin Polyclonal Antibody는 사람 및 마우스 시료에 반응하며, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합합니다. 액상 형태로 제공되며, 4°C에서 보관합니다. 췌장의 베타세포 염색에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 17 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 | 4 |
| Flow Cytometry (Flow) | - | 2 |
| ELISA | - | 1 |
| Functional Assay | - | 1 |
| Miscellaneous (PubMed) | - | 2 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Guinea pig / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full length human protein (Insulin, UniProt ID: P01308-1, antigen range: 1–110) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_794668 |
Product Specific Information
This antibody stains beta (β) cells of the Langerhans islets in human pancreas.
Note: Prolonged fixation in buffered formalin can destroy the epitope.
Target Information
Insulin is a peptide hormone secreted by β-cells of the pancreatic islets. It regulates carbohydrate, protein, and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It promotes glycogen storage, triglyceride formation, and synthesis of proteins and nucleic acids.
Insulin is a 51-amino acid polypeptide produced from the precursor peptide proinsulin, which is cleaved into two chains (A and B) linked by disulfide bonds and one molecule of C-peptide.
Deficiency of insulin results in diabetes mellitus, a major cause of morbidity and mortality. Insulin is also present in β-cell–derived tumors such as insulinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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