Thermo Fisher Scientific Peroxidase Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:900–1:4,000

ELISA

• Tested Dilution: 1:9,000–1:40,000

Immunoprecipitation (IP)

• Tested Dilution: 1:900–1:4,000

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as undiluted liquid.
• Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Peroxidase (Horseradish). Cross-reactivity against peroxidase from other tissues and species may occur but has not been specifically determined.

Target Information

Horseradish Peroxidase (HRP) is an enzyme widely used in biochemistry and molecular biology. Derived from the horseradish root, it catalyzes oxidation reactions in the presence of hydrogen peroxide. HRP is commonly utilized as a detection enzyme in immunoassays such as ELISA and Western blot due to its high sensitivity and stability. It also serves as a tracer in enzyme-linked assays and as a labeling agent in fluorescence microscopy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.