Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), Alexa Fluor 488, eBioscience
FOXP3 단백질을 인식하는 FJK-16s 클론의 Alexa Fluor 488 결합 단일클론 항체로, 마우스 및 다양한 종에서 Treg 세포 분석에 사용됩니다. Flow cytometry에 최적화되어 있으며 0.25 µg/test로 권장됩니다. 4°C에서 암소 보관하며 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 42 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Cat, Mouse, Pig, Rat |
| Published Species | Bacteria, Bovine, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FJK-16s |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469916 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- Biotin
- Brilliant Violet 421
- Brilliant Violet 605
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- eFluor 506
- eFluor 570
- eFluor 615
- eFluor 660
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine, and cat Foxp3 (FORKHEAD BOX P3, SCURFIN, JM2). Foxp3 is a 49–55 kDa transcriptional regulator involved in Treg cell function. The antibody epitope maps to amino acids 75–125 of mouse Foxp3. In humans, this region is alternatively spliced, whereas in mice it is not, indicating species-specific regulation.
FJK-16s is optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (Product #00-5523-00). Other fixation and staining buffers are not recommended.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Intracellular staining and flow cytometry of mouse splenocytes using the Foxp3/Transcription Factor Buffer Set.
Recommended Usage: ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration recommended for optimal results.
Excitation: 488 nm
Emission: 519 nm
Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is highly expressed in CD25⁺CD4⁺ regulatory T cells and absent in CD4⁻/CD8⁺ T cells. FOXP3 mutations cause the scurfy phenotype in mice and IPEX syndrome in humans. It is also regulated by p53 in response to DNA damage in human breast and colon cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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