Thermo Fisher Scientific Gpr161 Monoclonal Antibody (1A8)

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

1A8

Immunogen

Recombinant Mouse Gpr161

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer. Its the key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. It is recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, which leads to PKA-dependent processing of GLI3 into GLI3R and represses the Shh signaling. In presence of SHH, its removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling.

Target Information

Upon ligand binding, G protein-coupled receptors, such as GPR161, activate cytoplasmic G proteins (see GNAS, MIM 139320), allowing the receptors to transduce extracellular signals across the plasma membrane into the cell.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.