Thermo Fisher Scientific Human RPLPO (Large Ribosomal Protein) Endogenous Control (FAM/MGB probe, non-primer limited), 500 Reactions

Applied Biosystems™ Human RPLPO (Large Ribosomal Protein) Endogenous Control

(FAM™/MGB Probe, Non-Primer Limited)

The Applied Biosystems™ Human RPLPO (large ribosomal protein) Endogenous Control (FAM™/MGB Probe, Non-Primer Limited) is designed as an endogenous control for relative gene expression quantification in cDNA samples when used with other gene expression assays. The probe is labeled with 6FAM™ dye and MGB, and the primers are not limited. Suitable for singleplex PCR reactions.

This control is compatible with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and/or Custom TaqMan™ Primers and Probes.

Assay Details

TaqMan™ Endogenous Controls

TaqMan™ Endogenous Controls eliminate the need for assay design and optimization, providing pre-designed probe and primer sets to normalize RNA or DNA quantities in reactions. These controls simplify gene expression quantification across human, mouse, rat, and other eukaryotic genes.

Simple to Use

All components are QC tested and formulated into a single 20X mix, compatible with universal conditions for two-step RT-PCR. Use with TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and cDNA samples for sensitive, reproducible, and quantitative results on Applied Biosystems instruments such as 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000/7700 Sequence Detection Systems.

Flexible Offering

Each endogenous control is built using proven 5′ nuclease chemistry. Options include:

• FAM™ dye-labeled TaqMan™ MGB probe (250 nM final) and two unlabeled primers (900 nM each)
• VIC™ dye-labeled TaqMan™ MGB probe (250 nM final) and two unlabeled primers (150 nM each, primer limited)
• VIC™ dye-labeled TAMRA™ probe (250 nM final) and two unlabeled primers (150 nM each, primer limited)

Choosing the Right Endogenous Control

Endogenous controls normalize target gene expression by correcting for cDNA input differences. Verify consistent expression across all samples. For multiplexing, ensure the control gene is more abundantly expressed than the target.

Multiplex vs. Singleplex PCR

• VIC™-labeled controls are primer limited, enabling multiplexing.
• FAM™-labeled controls are non-primer limited, suitable for singleplex reactions.

For Research Use Only. Not for use in diagnostic procedures.

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