Thermo Fisher Scientific Eukaryotic 18S rRNA Endogenous Control (FAM/MGB probe, non-primer limited), 500 Reactions

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB Probe, Non-Primer Limited)

The Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB Probe, Non-Primer Limited) is intended as an endogenous control for relative gene expression quantification in cDNA samples when used with other gene expression assays.
Probe is labeled with 6FAM™ dye - MGB, and the primers are not limited.
Can be used for singleplex PCR reactions.
Compatible with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and/or Custom TaqMan™ Primers and Probes.

Assay Details

TaqMan™ Endogenous Controls

TaqMan™ Endogenous Controls eliminate the need for assay design, formulation, and testing by providing pre-designed probe and primer sets for normalization of RNA or DNA amounts in reactions.

Complete Solution for Quantitative Gene Expression

TaqMan™ Endogenous Controls simplify the selection of appropriate controls for common human, mouse, rat, and eukaryotic genes, reducing trial-and-error in gene expression quantification.

Simple to Use

All components are QC tested, formulated into a single 20X mix, and functionally validated.
Compatible with universal conditions for two-step RT-PCR.
Use with TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and your cDNA sample to generate sensitive, reproducible quantitative data on Applied Biosystems instruments such as 7900HT, 7300, 7500 Real-Time PCR Systems, and 7000/7700 Sequence Detection Systems.

Flexible Offering

Each endogenous control uses 5′ nuclease chemistry. Options include:

• FAM™ dye-labeled TaqMan™ MGB probe (250 nM final) with two unlabeled primers (900 nM each)
• VIC™ dye-labeled TaqMan™ MGB probe (250 nM final) with two unlabeled primers (150 nM each, primer limited)
• VIC™ dye-labeled TAMRA™ probe (250 nM final) with two unlabeled primers (150 nM each, primer limited)

Choosing the Right Endogenous Control

Endogenous controls normalize target gene expression by correcting for cDNA input differences.
Ensure consistent expression across all samples.
For multiplexing, the endogenous control gene should be more abundantly expressed than the target.

Multiplex vs. Singleplex PCR

• VIC™-labeled probes: primer limited, suitable for multiplexing.
• FAM™-labeled probes: non-primer limited, intended for singleplex use.

For Research Use Only. Not for use in diagnostic procedures.

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