Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Secondary Antibody, NovaFluor Blue 610-70S, eBioscience

Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Secondary Antibody, NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: Assay-dependent

Product Specifications

Additional Conjugate Formats

• AP
• Biotin
• FITC
• HRP
• NovaFluor Blue 510 / 555 / 585 / 660-120S
• NovaFluor Yellow 590 / 610 / 660
• PE
• Rhodamine
• TRITC
• WesternDot 625
• Custom conjugation available

Product Specific Information

This affinity-purified Goat Anti-Rat IgG (H+L) antibody exhibits minimal cross-reactivity to Mouse, Human, Bovine, Horse, and Rabbit serum proteins. Reacts specifically with heavy chains on rat IgG and light chains common to most rat immunoglobulins. No reactivity detected against non-immunoglobulin serum proteins.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for immunoblotting (WB), immunohistochemical staining, and ELISA.
Applications Tested: Verified by ELISA and/or solid-phase adsorption to minimize cross-reaction.

Suggested Dilution Ranges:

• Immunohistochemistry: 1:500 – 1:5,000
• ELISA / Western blot (chromogenic): 1:5,000 – 1:100,000
• Western blot (ECL): 1:10,000 – 1:200,000

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays with NovaFluor dyes.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), optimized for NovaFluor staining. Add 5 µL per sample to antibody cocktails before combining with cells. For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, engineered fluorescent signatures designed to minimize spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

Anti-Rat secondary antibodies are affinity-purified and specific for rat immunoglobulins. Ideal for detection, sorting, or purification of rat IgG targets. Secondary antibodies enhance assay versatility and sensitivity via multiple binding sites per primary antibody. Typically generated by immunizing host animals with pooled rat immunoglobulins and purified using immunoaffinity chromatography.

For Research Use Only. Not for diagnostic use or resale without authorization.

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