Thermo Fisher Scientific Phospho-EGFR (Ser1071) Polyclonal Antibody
EGFR 단백질의 Ser1071 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫에서 반응하며 고순도 정제 및 안정적 저장용 PBS/glycerol buffer 포함.
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Thermo Fisher Scientific Phospho-EGFR (Ser1071) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human EGFR (Accession P00533), corresponding to amino acid residues around phosphorylated Ser1071 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816200 |
Product Specific Information
This antibody detects endogenous levels of EGFR only when phosphorylated at Serine 1071.
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates cellular signaling in response to various growth factors. Overexpression of EGFR has been associated with numerous cancers and is targeted by both biological and small molecule therapeutics.
EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ERbB family, which includes ERbB2, ERbB3, and ERbB4.
Upon ligand binding (e.g., EGF or TGF-α), EGFR undergoes dimerization and autophosphorylation of tyrosine residues in its cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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