Thermo Fisher Scientific NAPE PLD Polyclonal Antibody
Rabbit polyclonal antibody against human NAPE-PLD, validated for IHC(P) and ICC/IF. Recognizes recombinant human NAPEPLD protein. Suitable for detecting neuronal expression and lipid signaling studies. Supplied as liquid, 0.3 mg/mL, stored at 4°C or -2...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NAPEPLD. Recombinant protein control fragment (Product # RP-92784) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644465 |
Product Specific Information
Immunogen sequence:
MKYQHVDPEE AVRIHTDVQT KKSMAIHWGT FALANEHYLE PPVKLNEALE
RYGLNAEDFF VLKHGESRYL NNDDEHighest antigen sequence identity to orthologs:
- Mouse: 85%
- Rat: 85%
Target Information
N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a membrane-bound enzyme responsible for releasing the endocannabinoid anandamide and other bioactive N-acylethanolamines (NAEs) from their corresponding NAPEs in animal tissues.
It is expressed by specific neuronal populations and targeted to axonal processes. NAEs generated by NAPE-PLD may act as anterograde synaptic signaling molecules regulating postsynaptic neuron activity.
NAPE-PLD also catalyzes the hydrolysis of NAPE to generate OEA (oleoylethanolamide), a lipid amide that suppresses food intake in rodents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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