Thermo Fisher Scientific EEA1 Monoclonal Antibody (B0-A8)
EEA1 단백질을 인식하는 Mouse monoclonal 항체로 Western blot 및 IHC(Paraffin)에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 기반 저장용액에 포함되어 있으며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B0-A8 |
| Immunogen | Synthetic peptide of the N-terminal residues of Human EEA1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931078 |
Target Information
Early endosomal antigen 1 (EEA1) is a 162 kDa membrane-bound protein specific to early endosomes and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments that fuse with endocytic vesicles for redistribution of extracellular compounds. EEA1 contains zinc-finger-like domains at both amino and carboxyl termini, similar to those found in nucleic acid binding proteins. The carboxyl-terminal zinc-finger-like domain is conserved in several other non-nuclear proteins involved in intracellular trafficking and is critical to EEA1 localization. Membrane association of EEA1 depends on phosphatidyl 3-kinase activity; inhibitors of this enzyme cause EEA1 to dissociate from early endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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