Thermo Fisher Scientific Pumilio 1 Polyclonal Antibody
Goat-derived polyclonal antibody against Human Pumilio 1 (PUM1) region 225–275. Suitable for Western blot and immunoprecipitation. Unconjugated, liquid form, 1 mg/mL concentration. Stored at 4°C and shipped on wet ice.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–10 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 225 and 275 of Human Pumilio 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
PUM1 (pumilio homolog 1) is a sequence-specific RNA-binding protein acting as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. It belongs to the PUF family of evolutionarily conserved RNA-binding proteins. PUM1 binds the RNA consensus sequence known as the Pumilio Response Element (PRE, 5'-UGUANAUA-3'), related to the Nanos Response Element (NRE).
PUM1 mediates post-transcriptional repression via different mechanisms, including recruitment of the CCR4-POP2-NOT deadenylase complex, leading to translational inhibition and mRNA degradation. Mutations in the PUM1 gene can result in spinocerebellar ataxia 47.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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