Thermo Fisher Scientific OspA Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

This antibody is specific for Borrelia burgdorferi OspA protein.
BLAST analysis suggests cross-reactivity with OspA from B. burgdorferi based on 100% homology with the immunizing sequence.
Cross-reactivity with OspA or Osp from other sources has not been determined.

Target Information

Outer-Surface Protein A (OspA) is a lipoprotein from Borrelia burgdorferi encoded on its plasmid lp54. It is a major component of the spirochete’s extracellular matrix and probably serves as a lipid-anchor.
During tick feeding, spirochetes migrate from the midgut to the salivary glands and are transmitted to the mammalian host.
This transition may be facilitated by changes in gene expression, including OspA.
Expression of spirochete proteins may be regulated by tick life cycle, feeding conditions (temperature, pH, nutrients), and infection progression in the mammalian host.
B. burgdorferi can attach to and differentially express antigens in diverse tissues within both vertebrate hosts and tick vectors, indicating that multiple physiological factors modulate gene expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.