Thermo Fisher Scientific p35 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

ELISA (ELISA)

>1:5,000

-

Immunoprecipitation (IP)

1:1,000

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MBP-fusion protein corresponding to Borrelia burgdorferi p35 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for Borrelia burgdorferi p35 protein. A BLAST analysis was used to suggest cross-reactivity with p35 from B. burgdorferi, garinii, and afzelii sources based on 100% homology with the immunizing sequence. Cross-reactivity with p35 from other sources has not been determined.

Target Information

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimers disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimers disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.