Thermo Fisher Scientific HYOU1 Recombinant Rabbit Monoclonal Antibody (201)
HYOU1 단백질을 인식하는 recombinant rabbit monoclonal antibody로, Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 사용 가능. 높은 특이성과 재현성을 제공하며, preservative-free 포뮬레이션으로 제공. 인간 ORP150/HYOU1/HSP12A에 특이적.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:20–1:100 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 1–4 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 201 |
| Immunogen | Recombinant Human ORP150/HYOU1/HSP12A protein (Met695–Leu994) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785218 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
This antibody has specificity for Human ORP150/HYOU1/HSP12A.
Target Information
HYOU1 belongs to the heat shock protein 70 family and plays an important role in protein folding and secretion in the endoplasmic reticulum (ER). Suppression of this protein is associated with accelerated apoptosis, suggesting a cytoprotective role under hypoxic conditions. HYOU1 is upregulated in tumors, particularly in breast cancer, and is linked to tumor invasiveness. This protein, lacking a signal peptide and only three amino acids shorter than the mature ER protein, may also have a housekeeping function in the cytosol. In rat, it localizes to both the ER and mitochondria through distinct targeting sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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